Bla bla bla RNA sequencing with single cell resolution
The workflow is as follows
-select tissue (link to HKDB page)
-cut tissue (link to tissue cutting guide)
-perform QC on extracted RNA (perform this step only, if sufficient amount of tissue is available)(link to FA)
-generate nuclei (nuclei preparation) or cells (link to cell dissociation)
-run Chromium (link to Chromium)
-pooling of libraries (link to pooling)
-sequencing (link to sequencing)
-hand over to dry lab section (appropriate dry lab link)
Upon completion of the single cell experiment workflow, you should have updated the following:
-Transfer the information from the google sheet (external link to sheet) and clean it
-Update HKDB: List of appropriate sheets
-Generate E-lab entries for: Library and sequencing
-Announce the completed (and demultiplexed) sequencing run in slack