In FG Kaessmann we use only induced pluripotent stem cells (iPSCs).
Here are the cell lines we have:
full name: SCTi003-A - a cell line bought by us from STEMCELL Technologies
in FG kaessmann?: yes
biosafety level: S1
GMO: yes (because →)
reprogramming method: plasmid or virus
cell bank created: yes
cultured in: mTESR plus, no antibiotics
cultured on: Matrigel
passaged with: ReLeSR, 5 min 37C, no anti-apoptotic after
passaged in, ratio: clumps, 1:20-1:30
individual: female
cell line of origin: PBMC
original culturing protocol
cell line reference/registration:hPSCreg database entry
note: so far can't be used for further experiments. StemCell Technologies have to provide the info on reprogramming procedure.
IDP52555 (sometimes marked as ZNF492-WT or ZNF) - a cell line, shared with us by Michael Heide from Göttingen
in FG kaessmann?: yes
biosafety level: S1
GMO: yes (because →)
reprogramming method: virus, reprogramming kit, similar to the protocol
cell bank created: yes
cultured in: mTESR plus, no antibiotics
cultured on: Geltrex or Matrigel
passaged with: ReLeSR, 5 min 37C, no anti-apoptotic after
passaged in, ratio: clumps, 1:6-1:10
individual: female
cell line of origin: fibroblasts
original culturing protocol
cell line reference/registration: hPSCreg database entry
Repro 771-3G - a cell lines, shared with us by Lena Kutscher from DKFZ
in FG kaessmann?: yes
biosafety level: S1
GMO: no
reprogramming method: mRNA, StemRNA™ 3rd Gen Reprogramming Kit
cell bank created: not yet
cultured in: mTESR plus, no antibiotics
cultured on: Matrigel
passaged with: ReLeSR, 5 min 37C, no anti-apoptotic after
passaged in, ratio: clumps, 1:10-1:15
individual: male
cell line of origin: endothelial progenitor cells (from blood)
cell line reference/registration: ReproCell
JoC - a cell lines, shared with us by Svante Pääbo from MPI, Leipzig
in FG kaessmann?: yes
biosafety level: S2 (not tested for HIV)
GMO: no
reprogramming method: plasmids from Okita et al, 2013
cell bank created: not yet
cultured in: mTESR plus, no antibiotics
cultured on: Matrigel
passaged with: will add later
passaged in, ratio: will add later
individual: ?
cell line of origin: primary lymphocytes (from blood)
original culturing Leipzig cell culture protocol
cell line reference/registration: Kanton et al, 2019
Sandra - a cell lines, shared with us by Svante Pääbo from MPI, Leipzig
in FG kaessmann?: yes
biosafety level: S1
GMO: no
reprogramming method: plasmids from Okita et al, 2013
cell bank created: yes
cultured in: mTESR plus, no antibiotics
cultured on: Matrigel
passaged with: ReLeSR, 5 min 37C, no anti-apoptotic after
passaged in, ratio: clumps, 1:6-1:10
individual: ?
cell line of origin: leukocytes (from blood)
original culturing Leipzig cell culture protocol
cell line reference/registration: Mora-Bermúdez et al, 2016
Karlchen - a cell lines, shared with us by Sebastian Diecke from MDC, Berlin
in FG kaessmann?: yes
biosafety level: S2
GMO: yes
reprogramming method: virus, reprogramming kit
cell bank created: not yet
cultured in: E8 (mTESR on the vial), no antibiotics
cultured on: Matrigel
passaged with: EDTA/versene, X, X
passaged in, ratio: clumps, X
individual: male
cell line of origin: fibroblasts
cell line reference/registration: Wilson et al, 2020
Assumbo 1 PBMC - a cell lines, shared with us by Sebastian Diecke from MDC, Berlin
in FG kaessmann?: yes
biosafety level: S2
GMO: yes
reprogramming method: virus, reprogramming kit
cell bank created: not yet
cultured in: E8 (mTESR on the vial), no antibiotics
cultured on: Matrigel
passaged with: EDTA/versene, X, X
passaged in, ratio: clumps, X
individual: number 1, male, same as Assumbo 2
cell line of origin: PBMC
cell line reference/registration: Wilson et al, 2020
note: not QCed by MDC
Assumbo 2 fib - a cell lines, shared with us by Sebastian Diecke from MDC, Berlin
in FG kaessmann?: yes
biosafety level: S2
GMO: yes
reprogramming method: virus, reprogramming kit
cell bank created: not yet
cultured in: E8 (mTESR on the vial), no antibiotics
cultured on: Matrigel
passaged with: EDTA/versene, X, X
passaged in, ratio: clumps, X
individual: number 1, male, same as Assumbo 1
cell line of origin: fibroblasts
cell line reference/registration: Wilson et al, 2020
note: QC performed by MDC
a cell line, shared with us by Rüdiger Behr from Göttingen
in FG kaessmann?: yes
biosafety level: S1
GMO: yes
reprogramming method: plasmids transfection, cassette integrated, clone 33.1 (publication)
cell bank created: yes
cultured in: iPSC Brew, 1uM IWR1, 0.5uM CHIR, no antibiotics
cultured on: Geltrex
passaged with: versene, 3 min RT, add new versene, 3 min RT, add anti-apoptotic after passaging
passaged in, ratio: clumps, 1:10-1:15
individual: 002468 (DPZ number), male
original culturing protocol
cell line reference/registration: Stauske et al, 2020
A cell line, shared with us by Rüdiger Behr from Göttingen
in FG kaessmann?: yes
biosafety level: S1
GMO: yes
reprogramming method: plasmids transfection, no cassette integration, clone 24.4 (publication)
cell bank created: yes
cultured in: iPSC Brew, 1uM IWR1, 0.5uM CHIR, no antibiotics
cultured on: Geltrex
passaged with: versene, 3 min RT, add new versene, 3 min RT, add anti-apoptotic after passaging
passaged in, ratio: clumps, 1:10-1:15
individual: 002616 (DPZ number), female
original culturing protocol
cell line reference/registration: Stauske et al, 2020
cj_080619_4, cj_160919_5, cj_160919_6 - 3 cell lines, shared with us by Rüdiger Behr from Göttingen
in FG kaessmann?: yes
biosafety level: S1
GMO: no
reprogramming method: mRNA, clones 1-3 (publication)
cell bank created: no (waiting for hypoxia incubator)
cultured in: iPSC Brew, 1uM IWR1, 0.5uM CHIR, no antibiotics, hypoxia
cultured on: Geltrex
passaged with: versene
passaged in, ratio: clumps, 1:10-1:15
individual: 018008; 017811; 017812 (DPZ number), gender ?
original culturing will add later
cell line registration: NA