Bla bla bla RNA sequencing with single cell resolution The workflow is as follows -select tissue (link to HKDB page)\\ -cut tissue (link to tissue cutting guide)\\ -perform QC on extracted RNA (perform this step only, if sufficient amount of tissue is available)(link to FA)\\ -generate nuclei ([[wetlab:single_cell:nuc_prep|nuclei preparation]]) or cells (link to cell dissociation)\\ -run Chromium (link to Chromium)\\ -pooling of libraries (link to pooling)\\ -sequencing (link to sequencing)\\ -hand over to dry lab section (appropriate dry lab link)\\ Upon completion of the single cell experiment workflow, you should have updated the following:\\ -Transfer the information from the google sheet (external link to sheet) and clean it\\ -Update HKDB: List of appropriate sheets\\ -Generate E-lab entries for: Library and sequencing\\ -Announce the completed (and demultiplexed) sequencing run in slack\\