====== HOW TO SEQUENCE ======

===== Guide from finished pool to starting the Gerda =====


  * Unfreeze the Pool  , PhiX ,Flowcell ,  HT1  and Reagent Cartridge4 (illumina).
  * HT1 and Reagent Cartridge can thawed in water to save some time.
  * Take 2 empty 1,5ml Tubes and dilute NaOH  (dilute to 0.2N) and Tris5 (dilute to 200 nM) with UltraPure Water  (total 100µl dilution). 
  * Take 2 empty 1,5 ml Tubes for Denaturation and final Dilution.
  * Denaturation: mix 5µl the ~ 4nM Pool and 5 µl freshly diluted 0.2N NaOH
  * Vortex briefly and then centrifuge at 280x g for 1 minute
  * incubate at room temperature for 5 minutes
  * add 5 µl Tris-HCl, pH 7
  * Vortex briefly and then centrifuge at 280x g for 1 minute
  * dilute denaturated Pool to 20 pM with prechilled HT1. 
  * How much HT1 you need depends on the pool conc. (see in Pooling sheet)
    Pool conc.:             _________________
    HT1-Buffer vol.:    _________________
  * Vortex briefly and then centrifuge at 280x g for 1 minute
  * Place the 20 pM pools on ice until you are ready to proceed to final dilution